老司机午夜精品99久久免费,国产色无码专区在线观看,动漫人物桶动漫人物免费观看网站,99精产国品一二三产区

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>動物卵巢細胞>CRL-9618CHO-K1 倉鼠卵巢細胞亞株

CHO-K1 倉鼠卵巢細胞亞株

簡要描述:CRL-9618 CHO-K1 倉鼠卵巢細胞亞株,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:CRL-9618
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2309

產(chǎn)品分類

Product Category

詳細介紹

CHO-K1 倉鼠卵巢細胞亞株

1957年,Puck TT從成年中國倉鼠卵巢的活檢組織建立了CHO細胞,CHO-K1是CHO的一個亞克隆。CHO-K1的生長需要

培養(yǎng)條件:F12K培養(yǎng)基(SIGMA,貨號N3520,添加NaHCO3 2.5g/L),90%;優(yōu)質(zhì)胎牛血清,10%


動物種別:中國倉鼠

性別:雌

組織來源:卵巢

形態(tài):上皮細胞

傳代方法的介紹   1:4-1:8

支原體檢測   陰性

以下是此細胞ATCC

CHO-K1 (ATCC® CCL-61™)
CHO-K1 倉鼠卵巢細胞亞株

Organism Cricetulus griseus, hamster, Chinese

Tissue  ovary

Product Format  frozen

Morphology  epithelial-like

Culture Properties  adherent

Biosafety Level  1

Gender  female

Applications

This cell line is suitable as a transfection host.

Storage Conditions  liquid nitrogen vapor phase

Karyotype  Chromosome Frequency Distribution 50 Cells: 2n = 22. Stemline number is hypodiploid.

Derivation

The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957.

Clinical Data

female

Virus Susceptibility  Vesicular stomatitis, Orsay (Indiana)

Vesicular stomatitis, Glasgow (Indiana)

Getah virus

Virus Resistance

poliovirus 2; modoc virus; Button Willow virus

Comments

The cells require proline in the medium for growth.

Complete Growth Medium  The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w

) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: Once or twice between subculture

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
嫩bbb槡bbbb槡bbbb| 一边摸一边叫床一边爽| 入禽太深免费完整版电影| 最近免费韩国电影高清版无吗| 高中女厕原味卫生巾巾5565| 久久无码人妻一区二区三区| 亚洲AV色香蕉一区二区| 特黄A片在线播放免费麻婆豆腐| 年轻的馊子6中字HD| 四虎成人精品在永久免费 | 国产精品国产三级国产AV中文| 国产av一区二区三区日韩| 国产精品情侣呻吟对白视频| 乱暴tubesex中国妞| 亚洲国产精品一区二区久久hs | 同性男在啪的动漫网站| 久久久久99精品成人片欧美| 在教室伦流澡到高潮hnp| 又粗又长又大真舒服好爽| 大香伊在人线免| 特黄特色大片免费播放器下载| 人妻丰满熟妇AV无码区不卡| 18禁美女裸身无遮挡免费网站 | 欧美 校园 激情 清纯 另类| 国产一区二区三区精品| 免费1级a做爰片观看| 国产毛多水多女人a片| 欧美性猛交xxxx乱大交| 无遮真人祼交视频| 少妇被猛烈挺进爽爽a片小说| 在线观看的资源视频| 亚洲精品无码乱码成人| 久久精品99国产精品日本| 97免费人妻在线视频| 少妇与公做了一夜伦理| 狠狠躁18三区二区一区| YY6080韩国三级理论无码| 自由 日本语 热 亚洲人| 在线免费黄色电影| 99久久久无码国产精品免费砚床| 国产羞羞视频在线观看播放|